| Specificity |
GenScript RFP-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing RFP-tags and RFP variants, such as mCherry, tdTomato, DsRed, DsRed2, mOrange.The antibody does not react with GFP or GFP-tagged proteins. |
| Host Species |
Rabbit |
| Immunogen |
RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated to KLH |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.05-0.2 µg/ml |
| Western Blot |
0.1-1.0 µg/ml |
| Western Blot |
1:100-1:1,000 |
|
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Storage Instructions |
The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
Loading: RFP-tag fusion protein expressed in E. coli cell lysate
Primary antibody: 1 μg/ml Rabbit Anti-RFP-tag Polyclonal Antibody (GenScript, A00682)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204).
Western blot analysis of red fluorescent protein variants (mCherry, tdTomato, DsRed, DsRed2, mOrange)
using RFP-tag Antibody, pAb, Rabbit (GenScript, A00682, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Lane 1: Marker
Lane 2-5: 40 ng, 8 ng, 1.6 ng, 0.8 ng RFP-tag fusion protein expressed in E. coli cell lysate
Primary antibody: 1 μg/ml Rabbit Anti-RFP-tag Polyclonal Antibody (GenScript, A00682)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG
| Target Background |
Well-characterizated antibodies against short-sequence epitope tags are common in the study of protein expression in systems.RFP-tags are composed of 15-residue peptides, VNGHEFEIEGEGEGR, derived from amino acids 22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone. |
| Synonyms |
Rabbit Anti RFP-tag pAb;Anti RFP-tag pAb |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
