| Specificity |
GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody specifically recognizes V5 tagged fusion proteins. |
| Host Species |
Rabbit |
| Immunogen |
V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH |
| Conjugate |
Horseradish peroxidase (HRP) |
|
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.05-0.2 µg/ml |
| Western Blot |
0.1-1.0 µg/ml |
|
| Form |
Lyophilized powder |
| Storage Buffer |
1×PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 1 mg/mL. |
| Storage Instructions |
The product remains stable for up to 1 year at -20 °C from the date of receipt. The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
| Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.Before use, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap. |
![V5-tag Antibody [HRP], PAb, Rabbit](/product/documents/down?doc_name=3634_20071206011610.JPG&file=scm_files/productFile_notes/2023/11/14/b39e38e7-cce1-4a4b-94d4-5f0b0a4f2d4d.JPG "V5-tag Antibody [HRP], PAb, Rabbit")
Western blot analysis of V5-tagged fusion protein using V5-tag Antibody [HRP], pAb, Rabbit (GenScript, A00877, 1 µg/ml)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD
| Target Background |
GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase.GenScript Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags. |
| Synonyms |
Rabbit Anti-V5-tag [HRP] pAb; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
