| Specificity |
Recombinant Cre recombinase from E.coli expression systems. |
| Host Species |
Mouse |
| Immunogen |
Purified recombinant Cre recombinase protein |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator.The chart below may serve as a useful guideline, but secondary antibody affinity, antigen concentration, temperature, incubation time, and the sensitivity of the detection methods can all affect the ideal working concentration.We have not yet determined the suitability of this antibody for other applications.The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.05-0.2 µg/ml |
| Western Blot |
0.1-1.0 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Storage Instructions |
The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG2a |
| Clonality |
Monoclonal |
| Clone ID |
1D8H3 |
Loading: Cre recombinase protein
Primary antibody: 1 μg/ml Mouse Anti-Cre Recombinase Monoclonal Antibody (GenScript, A00888)
Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)
| Target Background |
Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage.It is highly specific to a 34 bp DNA sequence (loxP) found in P1 DNA.It catalyzes site-specific recombination between two 34-base-pair LOXP sites.Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state.It is homotetramer when bound to DNA and belongs to the phage integrase family.GenScript Mouse Anti-Cre recombinase Monoclonal Antibody is an important tool for the identification of recombinant Cre recombinase from E.coli expression systems. |
| Synonyms |
anti-Cre recombinase mAb; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
