| Specificity |
THE™ alpha Tubulin Antibody, mAb, Mouse reacts with mouse, human, hamster, monkey, and pig α-tubulin. This product has not yet been tested in other species. |
| Host Species |
Mouse |
| Immunogen |
A synthetic peptide (ALEKDYEEVGVDSVE) coupled to KLH |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.05-0.2 µg/ml |
| Western Blot |
0.1-1.0 µg/ml |
| Immunocytochemistry/Immunofluorescence (ICC/IF) |
1 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, ti can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG2b,κ |
| Clonality |
Monoclonal |
| Clone ID |
1F4E3 |
| Note |
GenScript can offer this product per customer's reuqest including product size, buffer components,etc. |
Immunofluorescence analysis of 293 cells
Primay antibody: 1 μg/ml THETM Anti-alpha tubulin Monoclonal Antibody (Mouse) (GenScript, A01410)
Secondary antibody: Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (H&L) (Goat) (Rockland, 1:1,000, 610-102-121)
Western blot analysis of cell and tissue lysates using THETM alpha Tubulin Antibody, mAb, Mouse (GenScript, A01410, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Predicted Size: 50 kD
Observed Size: 50 kD
Comparison of THETM alpha Tubulin Antibody, mAb, Mouse (GenScript, A01410) with Mouse Anti-alpha Tubulin mAb (Competitor A) by Western blot.
A: THETM alpha Tubulin Antibody, mAb, Mouse (GenScript, A01410, 1 µg/ml)
B: Mouse Anti-alpha Tubulin Monoclonal Antibody (Clone: DM1A, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Predicted Size: 50 kD
Observed Size: 50 kD
| Target Background |
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Microtubules are obligate proteinaceous elements found in nearly all eukaryotic cells. The availability of monoclonal antibodies to α-tubulin uses as a common housekeeping protein and serves as a specific and useful tool in studying proper interpretation of western blots. |
| Synonyms |
α-tubulin mAb; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
