| Specificity |
GenScript MonoRab™ DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit specifically recognizes DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of DYKDDDDK fusion proteins. |
| Host Species |
Rabbit |
| Immunogen |
DYKDDDDK synthetic peptide coupled to KLH |
| Conjugate |
Biotin |
|
Working concentrations for specific applications should be determined by the investigator. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.2-0.5 μg/ml |
| Western Blot |
1 μg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Rabbit IgG |
| Clonality |
Monoclonal |
| Clone ID |
114F12C8 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
![MonoRab™ DYKDDDDK Tag Antibody [Biotin], MAb, Rabbit](/product/documents/down?doc_name=A01870_binding.JPG&file=scm_files/productFile_notes/2017/08/02/7beb0315ee0f88a01419a50bc8982a16.JPG "MonoRab™ DYKDDDDK Tag Antibody [Biotin], MAb, Rabbit")
MonoRabᵀᴹ DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit (GenScript, A01870-40) binds with DYKDDDDK Tag.
Coating antigen: streptavidin, 5 µg/ml.
MonoRabᵀᴹ DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit (GenScript, A01870-40) dilution start from 1,000 ng/ml.
Secondary Antibody: Mouse Anti-Rabbit IgG Antibody (M205) [HRP], mAb
EC50= 83.47 ng/ml.
![MonoRab™ DYKDDDDK Tag Antibody [Biotin], MAb, Rabbit](/product/documents/down?doc_name=A01870_WB2.JPG&file=scm_files/productFile_notes/2017/08/02/38c8285fd23c4669e2c92cc8cacb407e.JPG "MonoRab™ DYKDDDDK Tag Antibody [Biotin], MAb, Rabbit")
Sensitivity and specificity of MonoRabᵀᴹ DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit (GenScript, A01870-40) to N-DYKDDDDK-tag Fusion Protein by Western blot.
Loading:
Lane 1 : N-terminal DYKDDDDK-tagged fusion protein (25 ng)
Lane 2 : N-terminal DYKDDDDK-tagged fusion protein (10 ng)
Lane 3 : N-terminal DYKDDDDK-tagged fusion protein (5 ng)
Lane 4 : N-terminal DYKDDDDK-tagged fusion protein (1 ng)
Primary Antibody:
MonoRabᵀᴹ DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit (GenScript, A01870-40) 1 μg/ml
Secondary Antibody:
Streptavidin-HRP(M00091,15L000972) 0.2 μg/ml
![MonoRab™ DYKDDDDK Tag Antibody [Biotin], MAb, Rabbit](/product/documents/down?doc_name=A01870_WB1.JPG&file=scm_files/productFile_notes/2017/08/02/f43a1d9d4d2a3c5c21289e22b37c1cc4.JPG "MonoRab™ DYKDDDDK Tag Antibody [Biotin], MAb, Rabbit")
Sensitivity and specificity of MonoRabᵀᴹ DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit (GenScript, A01870-40) to C-DYKDDDDK-tag Fusion Protein by Western blot.
Loading:
Lane 1 : C-terminal DYKDDDDK-tagged fusion protein (25 ng)
Lane 2 : C-terminal DYKDDDDK-tagged fusion protein (10 ng)
Lane 3 : C-terminal DYKDDDDK-tagged fusion protein (5 ng)
Lane 4 : C-terminal DYKDDDDK-tagged fusion protein (1 ng)
Primary Antibody:
MonoRabᵀᴹ DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit (GenScript, A01870-40) 1 μg/ml
Secondary Antibody:
Streptavidin-HRP(M00091,15L000972) 0.2 μg/ml
| Target Background |
The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRab™technology to generate the high affinity and specificity monoclonal rabbit antibodies. MonoRab™ DYKDDDDK Tag Antibody [Biotin], mAb, Rabbit specific to DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of fusion proteins. The antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify DYKDDDDK fusion proteins in bacteria and mammalian cells. |
| Synonyms |
Rabbit monoclonal to Flag |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
