| Specificity |
The product is specific for Daratumumab. |
| Host Species |
Rabbit |
| Immunogen |
Daratumumab |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
0.01-0.1 µg/ml |
| Competitive ELISA |
1-10 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Storage Instructions |
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Rabbit IgG |
| Clonality |
Monoclonal |
| Clone ID |
139B10 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
ELISA binding of MonoRabTM Anti-Daratumumab Antibody (139B10) (GenScript, A02009-40) with Daratumumab. While the antibody does not recognize the human IgG Fc fragment (data not shown). Coating antigen: Daratumumab, 1 µg/ml. MonoRabTM Anti-Daratumumab Antibody (139B10) (GenScript, A02009-40) dilutions start from 1,000 ng/ml. EC₅₀= 3.092 ng/ml.
MonoRabTM Anti-Daratumumab Antibody (139B10), mAb, Rabbit (GenScript, A02009-40) blocks Daratumumab binding with Human CD38 protein. Coating antigen: CD38 protein, 1 µg/ml Daratumumab-biotin final concentration: 10 ng/ml MonoRabTM Anti-Daratumumab Antibody (139B10), mAb, Rabbit (GenScript, A02009-40) dilutions start from 10 μg/ml IC₅₀= 17.11 ng/ml
ELISA binding of MonoRabTM Anti-Daratumumab Antibody (139B10) (GenScript, A02009-40) with Daratumumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Daratumumab, 1 µg/ml.
MonoRabTM Anti-Daratumumab Antibody (139B10) (GenScript, A02009-40) dilutions start from 1,000 ng/ml.
EC₅₀= 3.092 ng/ml.
| Target Background |
Daratumumab (Darzalex) is a human IgG1k monoclonal antibody that is approved by the U.S. Food and Drug Administration for the treatment of multiple myeloma. It binds with high affinity to CD38, which is highly expressed on the surface of multiple myeloma cells. Daratumumab can make immune system to attack cancer cells, resulting in rapid tumor cell death through immunomodulatory effects. |
| Synonyms |
Rabbit monoclonal antibody to Darzalex |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
