| Specificity |
The product is specific for AAV2 capsids VP1, VP2 and VP3. |
| Host Species |
Mouse |
| Immunogen |
AAV2 |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| Western Blot |
1 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
Lyophilized with PBS, pH 7.2, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Storage Instructions |
The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Protein A affinity column |
| Isotype |
Mouse IgG2c, k |
| Clonality |
Monoclonal |
| Clone ID |
12C10 |
| Note |
GenScript can customize this product per customer's request including product size, buffer components, etc. |
Western Blot analysis of AAV2 Antibody (12C10), mAb, Mouse (GenScript, A02091) with different copy numbers of purified AAV2 samples.
Lane 1： 8×10^9 Copies of purified AAV2 loading amount
Lane 2： 4×10^9 Copies of purified AAV2 loading amount
Lane 3： 2×10^9 Copies of purified AAV2 loading amount
Lane 4： 8×10^8 Copies of purified AAV2 loading amount
Primary Antibody:
Lane 1-4 : AAV2 Antibody (12C10), mAb, Mouse (GenScript, A02091) 1 μg/ml
Secondary Antibody:
Goat Anti-Mouse IgG Antibody (H&L) [HRP], pAb 0.25 μg/ml
| Target Background |
Adeno-associated virus type 2 (AAV2) is a valuable vector for gene therapy. It is one member of the human parvovirus family which depends on co-infection with helper viruses such as adenovirus or herpes virus for efficient reproduction. AAV2 empty capsids are composed of three proteins, VP1, VP2 and VP3 with relative molecular masses of 87, 72 and 62 kDa, respectively. |
| Synonyms |
Adeno-associated virus serotype 2 Antibody (12C10), AAV2 Antibody |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
