目录产品 » MMAF Antibody (11B8), mAb, Mouse

ELISA binding of MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) with MMAF, MMAE, DXd and DM1 conjugated antibody respectively.

The coating antibody was MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) used at 2 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including MMAF, MMAE, DXd and DM1 conjugated antibodies used at 1 μg/mL concentration. The secondary antibody was Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) used at 4 μg/ml concentration.

MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) is specific to MMAF and has no cross-reactivity with MMAE, DXd, and DM1.

Standard curve of MMAF Competitive ELISA.
The competitive ELISA assay is developed by using free MMAF block the binding of MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) to HRP conjugated MMAF.

Capture antibody: MMAF Antibody (11B8), mAb, Mouse, 2 μg/ml
Detection reagent: HRP conjugated MMAF, 10 μg/ml
Free MMAF dilution start from 2 μg/ml.

Standard curve of MMAF conjugated Pembrolizumab Sandwich ELISA by anti-Human IgG Fc antibody and A02225.
The Sandwich ELISA assay is developed by using MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) and Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) as the capture and detection antibodies, respectively.

In this ELISA assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.997.

Capture antibody: MMAF Antibody (11B8), mAb, Mouse, 2 μg/ml
Detection antibody: Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb, 4 μg/ml

Standard curve of MMAF conjugated Pembrolizumab Sandwich ELISA by anti-idiotype antibody and A02225.
The Sandwich ELISA assay is developed by using MMAF Antibody (11B8), mAb, Mouse (GenScript, A02225) and MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], mAb, Rabbit (GenScript, A01853) as the capture and detection antibodies, respectively.

In this ELISA assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.99974.

Capture antibody: MMAF Antibody (11B8), mAb, Mouse, 2 μg/ml
Detection antibody: MonoRab™ Anti-Pembrolizumab Antibody(90G12)[Biotin], mAb, Rabbit, 0.05 μg/ml

MMAF Antibody (11B8), mAb, Mouse

The product is specific for MMAF and MMAF structurally similar molecules.

产品编号	A02225
规格	40 μg 定制报价
数量
价格	￥1600


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产品概述

Specificity	The product is specific for MMAF and MMAF structurally similar molecules.
Host Species	Mouse
Immunogen	MMAF-KLH
Conjugate	Unconjugated

应用指导

Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.5-2 µg/ml
Sandwich ELISA	0.5-2 µg/ml
Competitive ELISA	0.5-2 µg/ml

产品属性

Form	Liquid
Storage Buffer	Supplied in PBS, pH 7.4, containing 0.02% ProClin300.
Concentration	0.5 mg/mL
Storage Instructions	The product remains stable up to 1 year at -20 °C from date of receipt. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG2b, κ
Clonality	Monoclonal
Clone ID	11B8A6
Note	GenScript can customize this product per customer's request including product size, buffer components, etc.

图例

技术背景

Target Background

Payloads are the important components of antibody-drug conjugates (ADCs). The most commonly used ADC payloads are MMAE, MMAF, DM1, and DXd. ADC pharmacokinetic (PK) studies include free payload analysis, conjugated antibody analysis, and total antibody analysis via ligand-binding assay (LBA) and LC-MS/MS assay. The anti-payload antibody is a useful reagent in PK assay for determining conjugated antibodies. It can be used as a capture antibody to determine the conjugated antibody in LBA assay or through immunocapture to enrich the ADC sample in LC-MS/MS assay. The MMAF, also known as Monomethylauristatin F, is a potent tubulin polymerization inhibitor. It is used as an antimitotic drug in the form of antibody drug conjugates (ADCs).

Synonyms

Monomethylauristatin F, Monomethyl Auristatin F, 745017-94-1

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.