BLI (Biolayer interferometry) binding affinity measurements of Anti-Teclistamab Antibody (10H1), mAb, Mouse (GenScript, A02253) to Teclistamab.

Teclistamab captured on HFC (Anti-Human IgG Fc) Probes can bind Anti-Teclistamab Antibody (10H1), mAb, Mouse with dissociation constant (KD) of 3.32E-10M.

Standard curve of Teclistamab sandwich ELISA by A02253 (10H1) and A02254 (36D3).

In this ELISA assay, Anti-Teclistamab Antibody (10H1), mAb, Mouse (GenScript, A02253) was coated at a concentration of 1 μg/ml, and Anti-Teclistamab Antibody (36D3), mAb, Mouse (GenScript, A02254) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/ml.
In this ELISA assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.99984. The typicaldynamic range of the assay is 1.56-100 ng/mL and its sensitivity of detecting Teclistamab is up to 1.56 ng/ml.

MRD analysis of the detection of Teclistamab in biological matrix.

The MRD is the minimum dilution necessary for the detection of Teclistamab in biological matrix with least interference. Serum samples from cynomolgus monkey were serially diluted to determine the MRD of this assay, and the test result suggestedthat MRD was as 1:10.
In this ELISA assay, Anti-Teclistamab Antibody (10H1), mAb, Mouse (GenScript, A02253) was coated at a concentration of 1 μg/ml, and Anti-Teclistamab Antibody (36D3), mAb, Mouse (GenScript, A02254) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/ml.

Specificity analysis of the detection of Teclistamab.

Teclistamab standard samples at 8 concentrations were spiked with 2 µg/mL of human IgG4. The test result demonstrated that the high concentration of human IgG4 did not interfere with the detection of Teclistamab.
In this ELISA assay, Anti-Teclistamab Antibody (10H1), mAb, Mouse (GenScript, A02253) was coated at a concentration of 1 μg/ml, and Anti-Teclistamab Antibody (36D3), mAb, Mouse (GenScript, A02254) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/ml.

Anti-Teclistamab Antibody (10H1) or (36D3) inhibits the binding of Teclistamab to Human CD3.
Coating antigen: Teclistamab 1 µg/ml.
Human CD3 final concentration: 3 ng/ml.
Anti-Teclistamab antibody dilutions start from 10 μg/ml.
Anti-Teclistamab Antibody (10H1), mAb, Mouse (GenScript, A02253) exhibited non-inhibitory properties, whereas Anti-Teclistamab Antibody (36D3), mAb, Mouse (GenScript, A02254) demonstrated significant inhibitory properties with an IC₅₀ value of 0.77 µg/ml.