BLI (Biolayer interferometry) binding affinity measurements of Anti-Abatacept Antibody (CT.F3), mAb, Mouse (GenScript, A02262) to Abatacept.
Anti-Abatacept Antibody (CT.F3), mAb, Mouse (GenScript, A02262) captured on MFC (Anti-Mouse IgG Fc) Probes can bind Abatacept with a dissociation constant (KD) of 1.71E-09M.
Standard curve and specificity analysis of the detection of Abatacept by A02262 (CT.F3) and A02263 (CT.E8).
Abatacept standard samples at 8 concentrations were spiked with 10 µg/mL of human IgG. The test result demonstrated that the high concentration of human IgG did not interfere with the detection of Abatacept.
In the ELISA assay shown in this chart, Anti-Abatacept Antibody (CT.F3), mAb, Mouse (GenScript, A02262) was coated at a concentration of 1 μg/ml, and Anti-Abatacept Antibody (CT.E8), mAb, Mouse (GenScript, A02263) conjugated with Biotin was used as a detection antibody at a concentration of 0.8 μg/ml. The same conclusion was reached when Anti-Abatacept Antibody (CT.E8), mAb, Mouse (GenScript, A02263) was used as a coated antibody at a concentration of 1 μg/ml and Anti-Abatacept Antibody (CT.F3), mAb, Mouse (GenScript, A02262) conjugated with Biotin was used as a detection antibody at a concentration of 0.8 μg/ml (data not shown)
In this assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square is greater than 0.99. The typicaldynamic range of the assay is 156-10,000 ng/ml and its sensitivity of detecting Abatacept is up to 156 ng/ml.
Standard curve and specificity analysis of the detection of Abatacept by A02262 (CT.F3) and anti-human IgG Fc antibody.
Abatacept standard samples at 8 concentrations were spiked with 2 µg/mL of human IgG. The test result demonstrated that the high concentration of human IgG did not interfere with the detection of Abatacept.
In the ELISA assay shown in this chart, Anti-Abatacept Antibody (CT.F3), mAb, Mouse (GenScript, A02262) was coated at a concentration of 1 μg/ml, and Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) was used as a detection antibody at a dilution of 1:2000.
In this assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square is greater than 0.99. The typicaldynamic range of the assay is 31.25-2,000 ng/ml and its sensitivity of detecting Abatacept is up to 31.25 ng/ml.
MRD analysis of the detection of Abatacept in biological matrix by A02262 (CT.F3) and A02263 (CT.E8).
The MRD is the minimum dilution necessary for the detection of Abatacept in biological matrix with least interference. Serum samples from cynomolgus monkey were serially diluted to determine the MRD of this assay, and the test result suggestedthat MRD was as 1:10.
In the ELISA assay shown in this chart, Anti-Abatacept Antibody (CT.F3), mAb, Mouse (GenScript, A02262) was coated at a concentration of 1 μg/ml, and Anti-Abatacept Antibody (CT.E8), mAb, Mouse (GenScript, A02263) conjugated with Biotin was used as a detection antibody at a concentration of 0.8 μg/ml.
In this MRD analysis, the same conclusion was reached when Anti-Abatacept Antibody (CT.E8), mAb, Mouse (GenScript, A02263) was used as a coated antibody at a concentration of 1 μg/ml and Anti-Abatacept Antibody (CT.F3), mAb, Mouse (GenScript, A02262) conjugated with Biotin was used as a detection antibody at a concentration of 0.8 μg/ml (data not shown).
MRD analysis of the detection of Abatacept in biological matrix by A02262 (CT.F3) and anti-human IgG Fc antibody.
The MRD is the minimum dilution necessary for the detection of Abatacept in biological matrix with least interference. Serum samples from cynomolgus monkey were serially diluted to determine the MRD of this assay, and the test result suggestedthat MRD was as 1:10.
In the ELISA assay shown in this chart, Anti-Abatacept Antibody (CT.F3), mAb, Mouse (GenScript, A02262) was coated at a concentration of 1 μg/ml, and Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) was used as a detection antibody at a dilution of 1:2000.
Anti-Abatacept Antibody (CT.F3), mAb, Mouse
A02262 | |
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¥990 | |
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