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Benz-Neburase™ GMP, Tag Free

Figure 2: Benz-NeburaseTM Nuclease reduces viscosity of bacterial lysate by digesting nucleic acid. 2.5 U Benz-Neburase™ GMP, tag free (Cat. No. Z03708) was used to treat 1 ml of bacterial lysate. Incubate at 37 °C for 30 min and then centrifuge the samples and observe the viscosity of the precipitate and the supernatant. The test results show that Benz-Neburase™ GMP, tag free can greatly reduce the viscosity of bacterial lysate.

Benz-Neburase™ GMP, Tag Free

Figure 3: Benz-NeburaseTM Nuclease prevents and reduces cell clumping. The adhered cells were spread in a 24-well plate and treated with control buffer (left) and 50 U/ml Benz-Neburase™ GMP, tag free (Cat. No. Z03708) (right) at 37 °C for 30 min. After the incubation, the cells were observed under a microscope. The test result show that Benz-Neburase™ GMP, tag free can efficiently reduce cell clumping.

Benz-Neburase™ GMP, Tag Free

Figure 4: Benz-NeburaseTM Nuclease digests various types of nucleic acids. In a 20 μl reaction volume, 1 U of Benz-Neburase™ GMP, tag free (Z03708) was used to digest different kinds of nucleic acids (PCR product, genomic DNA, plasmid DNA, and RNA) at 37 °C for 10 min. The test results show that Benz-Neburase™ GMP, tag free is effective in digesting various forms of DNA and RNA.

Benz-Neburase™ GMP, Tag Free

Figure 1: Benz-NeburaseTM Nuclease removes nucleic acid residues during AAV production. 100 U Benz-Neburase™ GMP, tag free (Cat. No. Z03708) was used to treat 2 ml of cell suspension at 37 °C for 60 min. Then the mix was centrifuged at 1600 g for 10 min to remove cells and cell debris, and measurements were taken for the HCD, plasmid DNA residues, and viral genome (Vg) recovery. The test results show that Benz-Neburase™ GMP, tag free can remove DNA and plasmid residue in AAV virus production process more effectively than the competing product. The usage of Benz-Neburase™ GMP, tag free has minimal impact on viral production.

Benz-Neburase™ GMP, tag free

Benz-Neburase™ GMP, tag free (Cat. No. Z03708) is manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards and with more stringent process controls and relatively complete document records.
Z03708
¥2100

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Product Introduction Benz-Neburase™ GMP, tag-free is a genetically engineered endonuclease from Serratia marcescens that cleaves all forms of DNA and RNA non-specifically under a broad range of conditions. Benz-Neburase™ Nuclease is mostly utilized to remove residual DNA and RNA during production process of biological molecules of interest in biopharmaceutical manufacturing including but not limited to vaccine production, viral vector production, as well as in gene and cell therapy-related manufacturing.
Benz-Neburase™ GMP, tag free (Cat. No. Z03708) is manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards and with more stringent process controls and relatively complete document records. The high-purity nucleases are suitable and fulfill the requirements for applications from basic research to clinical settings. 

Expression System E.coli
Species Serratia marcescens 
Tag Tag free
Theoretical Molecular Weight 27.0 kDa
Biological Activity 250 U/μl (or 1.1 × 106 U/mg)
One unit is defined as the amount of enzyme that digests sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C (within reaction volume 0.5 ml).
Storage Buffer 20 mM Tris-HCl, 2 mM MgCl2, 20 mM NaCl, 50% Glycerol, pH 8.0.
Storage & Stability Store at -20 °C for up to 24 months or at 4 °C for 2 weeks from the date of manufacture. Avoid repeated freeze-thaw cycles. Do not store below -20 °C!

Digests all forms of DNA and RNA (double stranded, single stranded, linearized, and circular forms) under a board range of conditions.

Appearance Clear, colorless liquid
Purity SEC-HPLC: ≥ 99%
Reducing SDS-PAGE: ≥ 95%
Endotoxin Level < 0.01 EU/μg
Residual HCP ≤ 20 ng/mg
Protease Activity Non-detectable
Bioburden < 1 CFU/ml
Mycoplasma Negative

  • Benz-Neburase™ GMP, Tag Free
  • Benz-Neburase™ GMP, Tag Free

    Figure 2: Benz-NeburaseTM Nuclease reduces viscosity of bacterial lysate by digesting nucleic acid. 2.5 U Benz-Neburase™ GMP, tag free (Cat. No. Z03708) was used to treat 1 ml of bacterial lysate. Incubate at 37 °C for 30 min and then centrifuge the samples and observe the viscosity of the precipitate and the supernatant. The test results show that Benz-Neburase™ GMP, tag free can greatly reduce the viscosity of bacterial lysate.

  • Benz-Neburase™ GMP, Tag Free
  • Benz-Neburase™ GMP, Tag Free

    Figure 3: Benz-NeburaseTM Nuclease prevents and reduces cell clumping. The adhered cells were spread in a 24-well plate and treated with control buffer (left) and 50 U/ml Benz-Neburase™ GMP, tag free (Cat. No. Z03708) (right) at 37 °C for 30 min. After the incubation, the cells were observed under a microscope. The test result show that Benz-Neburase™ GMP, tag free can efficiently reduce cell clumping.

  • Benz-Neburase™ GMP, Tag Free
  • Benz-Neburase™ GMP, Tag Free

    Figure 4: Benz-NeburaseTM Nuclease digests various types of nucleic acids. In a 20 μl reaction volume, 1 U of Benz-Neburase™ GMP, tag free (Z03708) was used to digest different kinds of nucleic acids (PCR product, genomic DNA, plasmid DNA, and RNA) at 37 °C for 10 min. The test results show that Benz-Neburase™ GMP, tag free is effective in digesting various forms of DNA and RNA.

  • Benz-Neburase™ GMP, Tag Free
  • Benz-Neburase™ GMP, Tag Free

    Figure 1: Benz-NeburaseTM Nuclease removes nucleic acid residues during AAV production. 100 U Benz-Neburase™ GMP, tag free (Cat. No. Z03708) was used to treat 2 ml of cell suspension at 37 °C for 60 min. Then the mix was centrifuged at 1600 g for 10 min to remove cells and cell debris, and measurements were taken for the HCD, plasmid DNA residues, and viral genome (Vg) recovery. The test results show that Benz-Neburase™ GMP, tag free can remove DNA and plasmid residue in AAV virus production process more effectively than the competing product. The usage of Benz-Neburase™ GMP, tag free has minimal impact on viral production.


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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