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Ni-charged MagBeads

The GenScript Ni-Charged MagBeads are developed for quick and efficient small-scale purification of polyhistidine-tagged proteins under native or denaturing conditions.
L00295
￥900

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Description

The GenScript Ni-Charged MagBeads are developed for quick and efficient small-scale purification of polyhistidine-tagged proteins under native or denaturing conditions.

Perform cell lysis under native or denaturing conditions. Add the cell lysate containing your polyhistidine-tagged proteins to the Ni-Charged MagBeads and allow the proteins bind to the MagBeads. Then the isolated proteins can be eluted from the beads. Magnetic separation eliminates the need for multiple tubes, minimizes the loss of sample and removes several steps of the centrifugation process. 
The GenScript Ni-Charged MagBeads are average 40 µm in size, super paramagnetic beads with strong metal-chelating agent covalently bound to their surfaces. They are pre-charged with nickel and ready to use for quick and small-scale purification of polyhistidine-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol and 1 mM NiSO4. The Ni-Charged MagBeads have a binding capacity of 40 mg 6×His-tagged protein per 1 mL settled beads (e.g. 4 mL 25% slurry).
Key Features
Key Features
  • Quick and convenient separation accomplished by magnetic force
  • High binding capacity with 40 mg of 6xHis-tagged protein/ml settled beads
  • Compatible with various reagents needed in the purification process (see table 1)
  • Can be regenerated for multiple uses
  • Extremely low nonspecific binding


Table 1 Reagents Compatible with Ni-charged Magnetic Beads

Denaturants Detergents Reducing agents Salts Others
6 M Gu•HCl 2% Triton X-100 20 mM β-ME 4 M MgCl2 50% glycerol
8 M Urea 2% Tween 20 1 mM DTT 5 mM CaCl2 20% ethanol
1% CHAPS 2 M NaCl 1 mM EDTA

Storage This product is stable until the expiration date stated on the COA, when stored unopened at 2–8°C. Do NOT freeze the product. Keep the MagBeads in liquid suspension during storage and all handling steps. Drying will cause loss of binding capacity and result in reduced performance. Resuspend the beads well before use. Be careful to avoid bacterial/fungal contamination. 


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