| Product Description |
Lipopolysaccharide (LPS) is a bacterial
endotoxin, and a major constituent of the cell walls of gram- negative
bacteria. During gram-negative bacteremia or endotoxemia, LPS is the principal
pathophysiological mediator by which bacteria can cause hypotension, organ
failure, disseminated intravascular coagulation, and fatal shock in mammalian
hosts. Removal of endotoxin from drugs, injectables, and other biological
products is necessary but can also be very difficult.
GenScript’s ToxinEraser™
is an endototoxin removal resin of high efficiency. It is based on affinity
matrix of modified polymyxin B (PMB), which allows highly efficient endotoxin
removal. The final endotoxin level can be decreased to less than 0.1 EU/ml with
repeated use of ToxinEraser™ endotoxin removal
resin. Final removal efficiency may vary depending on the sample type/source. |
| Key Features |
- High stability and high
removal efficiency
- High binding capacity: >
2, 000, 000 EU / ml (CV)
- Fast flow without any
constant speed pump
- Reusable up to five times
if properly regenerated
- Ready-to-use reagents and
materials, such as equilibration buffer, collection tubes, etc.
|
| Kit Contents |
| Kit Contents （Cat. No.:L00338） |
PK |
| ToxinEraser™ Endotoxin Removal Resin |
1.5 ml pre-packed column |
| Regeneration Buffer |
125 ml |
| Equilibration Buffer |
125 ml |
| Flow-Speed Control |
1 |
| Collection Tubes |
1 bag (3 Tubes) |
| Tips (1 ml) |
2 bags (6 Tips/bag) |
| Protocol |
1 |
|
| Product Information |
Characteristics of ToxinEraser™ Endotoxin
Removal Resin| Size | 1.5 ml in pre-packed
column | | Binding Capacity | Up to 2,000,000 EU/ml
resin | | Ligand | Modified PMB
(Polymixin B) | | pH Stability | pH 5 -10 | | Support Matrix | 4% cross-linked
agarose, spherical beads | | Mean Particle Size | 90 μm | | Storage | 2°C to 8°C (Do not
freeze.) | | Equilibration Buffer | Phosphate-Buffer, pH
8.0 | | Regeneration Buffer | Sodium Phosphate-Buffer, pH
8.0; Triton-114 | | Types of substances
that can be applied to the column | Purified recombinant proteins,
peptides, antibodies, polysaccharides, etc.
| | Tolerable Ionic Strength | 0.1 to 0.5 M NaCl | Tested reagents that do not
interfere with performance
| 20% DMSO, 20% ethanol, 20%
glycerol;1 M urea, 300 mM imidazole; 0.05% Tween 20, 10 mM DTT.
|
|
| Storage |
Regeneration and storage of the Column.
To regenerate the column, wash the column with 10 ml
of equilibration buffer and allow the column to drain completely. Add ethanol
to the regeneration buffer to a final concentration of 20%, adding at least 1.5
ml to the column for storage. Store at 2-8°C. Do not freeze.
|
For research and
manufacturing use. Direct human use, including taking orally and injection are forbidden.
