GenScript Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF Prepacked Column.
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.
| Cat. No. | Product Name
| Volume supplied
| Resin content
|
| L00680-12 | Protein A Resin FF (1mlx2) | 1mlx2 | 50% slurry |
| L00680-51 | Protein A Resin FF (5mlx1) | 5mlx1 | 50% slurry |
| L00680-55 | Protein A Resin FF (5mlx5) | 5mlx5 | 50% slurry |
Characteristics
| Ligand | Recombinant Streptococcal protein A expressed in E. coli |
| Number of IgG binding sites per ligand | 5 |
| M.W. of ligand | Approximately 34 kDa |
| PI of ligand | 5.17 |
| Degree of substitution | Approximately 5 mg protein A / ml settled resin |
| Static binding capacity | > 40 mg human IgG/ ml settled resin |
| Matrix component | Agarose, 4% highly cross-linked |
| Average particle size | 90 μm (45-165 μm) |
| Storage solution | 20% ethanol |
| Storage conditions | 2-8 °C |
| Shelf life | 18 months when stored unopened |
Column compatibility
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).
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