The GenCrispr Mutation Detection Kit provides a simple, reliable, and rapid method for the detection of site specific cleavage of genomic DNA that is extracted from cells transfected with constructs expressing engineered nucleases such as Transcription activator-like effector nucleases (TALEN), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9, or Zinc-finger nucleases (ZFN). The GenCrispr Mutation Detection Kit includes High-Fidelity DNA polymerase for amplifying the target regions from cells, and GenCrispr T7 Endonuclease I for recognizing and detecting the mismatches caused by gene editing tools. It provides an easy and reliable approach for estimating the efficiency of genome editing.
| Components |
Amount provided |
| 25-reaction kit |
100-reaction kit |
| High-Fidelity DNA polymerase |
10 μL |
40 μL |
| 5X PCR Reaction Buffer |
150 μL |
600 μL |
| GenCrispr T7 Endonuclease I () |
25 μL |
100 μL |
| 10X GenCrispr T7 Endonuclease I Reaction buffer |
50 μL |
500 μL |
| Control Template DNA |
30 μL |
120 μL |
| Control Primer Mix |
30 μL |
120 μL |
| Protease K |
25 μL |
100 μL |
| dNTP |
15 μL |
60 μL |
|
