The GenBuilder Plus Cloning Kit enables seamless and one-step cloning of multiple DNA fragments into a linearized vector. The proprietary enzyme mix fuses together DNA fragments efficiently and precisely by recognizing 15-40 bp overlaps at the end of each fragment. The system allows cloning of DNA fragments into any linearized vector, requires no pre-existing recombination sites or helper sequences, and eliminates the need for a complicated restriction and ligation process. The GenBuilder Plus Cloning kit can simultaneously clone 12 DNA fragments into a linearized vector. The kit can also be used to assemble single stranded DNA (ssDNA) oligos with double stranded DNA (dsDNA) fragments, and using ssDNA as a linker to fuse together two adjacent dsDNA fragments.