Lentivirus Titer p24 ELISA Kit

Genscript Lentivirus Titer p24 ELISA Kit allows researcher to quickly determine the titer of any HIV-1-based lentiviral supernatant using sandwich ELISA methods.

产品编号	L00938
规格	96 Tests 定制报价
数量
价格	￥4980


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产品概述

Product Description

GenScript’s Lentivirus Titer p24 ELISA kit (Cat. No. L00938) is a Sandwich ELISA designed for quantitatively measuring the physical titer of any HIV-1 based lentivirus in cell culture supernatant. For lentivirus purification and production, lentivirus titration is an essential characterization procedure for routine quality control and lot release, as well as for purification process optimization. This kit makes lentivirus titration easy, accurate and reproducible.
Lentivirus vector is derived from human immunodeficiency virus-1 (HIV-1). The vector, which contains a pseudotyped envelope with vesicular stomatitis virus envelope G (VSV-G) protein, has the ability to transfer gene(s) of interest into dividing and non-dividing cells. Many studies show that Lentiviral vectors have successfully delivered genes into neurons, lymphocytes, and macrophages. The vector is proven to be effective in transducing brain, liver, muscle, and retina in vivo without toxicity or immune responses. The vector is also used to integrate siRNA efficiently in a wide variety of cell lines.

产品简介

Linearity Range

31.25 pg/mL - 2000 pg/mL

Sensitivity

LoB: 14.13 pg/mL
LoD: 29.42 pg/mL
LoQ: 39.03 pg/mL

Precision

Intra-assay: CV ≤ 10%;
Inter-assay: CV ≤ 15%

Assay Temperature

25°C

Assay Time

1 hour 40 minutes

Kit Contents

Component	Quantity/Size	Part No.
Capture Plate	1 plate	E1-80
Standard Stock	1 vial (100 μL)	E1-10
Lysis Solution	1 vial (1.6 mL)	E1-A0
Biotin Anti-p24 Antibody	1 bottle (12 mL)	E1-20
Streptavidin-HRP	1 bottle (12 mL)	E1-30
TMB Solution	1 bottle (12 mL)	A1-40
20× Wash Solution	1 bottle (40 mL)	A1-70
Stop Solution	1 bottle (6 mL)	A1-50
Plate Sealer	2 pieces	N/A

Storage

The unopened kit is stable for at least 12 months from the date of manufacture at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

原理

Assay Principle

Lentivirus Titer p24 ELISA kit utilizes two HIV-1 p24 monoclonal antibodies which bind to different epitopes of the p24 protein. When standards or samples are added to the capture plate, the p24 protein in the sample can be captured on the plate coated with the HIV-1 p24 capture antibody. Then the HIV-1 p24 detection antibody conjugated with biotin is added to interact with the p24 protein bound on the plate. Streptavidin-Horseradish Peroxidase conjugate (Streptavidin-HRP) is added to interact with the biotin conjugated anti-p24 antibody (Biotin Anti-p24 Antibody). After washing steps, 3,3',5,5'-Tetramethylbenzidine solution (TMB Solution) is added resulting in formation of blue color. The reaction is stopped by adding Stop Solution. Application of the Stop Solution results in the color changing from blue to yellow. The intensity of the color can be read at 450 nm by a microplate reader.
The quantity of p24 protein in the sample is precisely quantified against a p24 standard curve. p24 values are then correlated to the lentivirus titer of packaging cell culture supernatants.