| Product Description |
MonoRab™ Rabbit Anti-Camelid VHH Antibody
Plate (Clear, 8×12 strip) (GenScript, L00946) is a
96-well microtiter plate coated with MonoRab™ Rabbit Anti-Camelid
VHH Cocktail (GenScript, A02014). It can bind camelid and humanized VHHs with
high specificity and capacity. The binding capacity and
detection limits of plate varies, depending on the amino acid constitution of
different VHH. The plate can be compatible with MonoRab™ Rabbit Anti-Camelid
VHH Cocktail [HRP] (GenScript, A02016), HRP (Horseradish
peroxidase) conjugated secondary antibody, or HRP-conjugated
specific antigen. Any detection reagent, which can
recognize Rabbit IgG, should not be applied to the plate, such as HRP-conjugated
Mouse anti-Rabbit IgG and HRP-Protein A.
|
| Pre-coated Antibody |
MonoRab™ Rabbit Anti-Camelid VHH Cocktail
|
| Sensitivity |
1 ng/ml
|
| Specificity |
Camelid (Alpaca and Llama) and humanized VHHs
|
| Binding capacity |
~3 ng/well
|
| Reagents compatibility |
Compatible with common used reagents (see Reagent Compatibility table)
|
| Storage |
MonoRab™ Rabbit Anti-Camelid
VHH Antibody Plate should be shipped on ice pack. The unopened plate is stable
for at least 1 years when stored at 2-8 ℃. The opened plate should
be used within one week.
|
There are several potential applications for the plate:
1. Fix anti-VHH antibodies on the plate, which provides a universal platform to monitor the VHH level in different culture condition and to detect specific VHH for immunogenicity studies.
2. High throughput screening of stable cell lines expressing camelid or humanized VHHs.
3. Study the pharmacokinetics of camelid or humanized VHH antibody drugs in serum.
4. Detect the immuno-response of the heavy-chain-only antibody in camelid after immunization. |
| Background |
Camelid VHH Antibody (also called single-domain antibody (sdAb), or Nanobody by Ablynx) is a peptide chain about 110 amino acids in length, containing one variable domain of a heavy-chain antibody (a VHH fragment). Like a whole antibody, the camelid VHH antibody is able to bind selectively to a specific antigen. Due to its small size, simple production and high affinity, the camelid VHH antibody has a broad range of applications in biotechnical and therapeutic research fields.
|
For research use only. Not for use in diagnostic
procedures
