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目录产品 » 稳定细胞系 » Human Recombinant CCKB Cholecystokinin Receptor Stable Cell Line
CHO-K1/CCKB Stable Cell Line

Figure 1: CCK8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CCKB cells. The cells were loaded with Calcium-4 prior to stimulation with the agonist, CCK8. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of CCK8 (Mean ± SD, n = 2). The EC50 of CCK8 on this cell was 0.95 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/CCKB Stable Cell Line

Cholecystokinins (CCK) are derived from preprocholecystokinin. They are widely expressed in gastrointestinal tissues and in the CNS. There are two cholecystokinin receptors, referred to as A and B (CCKAR and CCKBR). CCKBR can be activated by the smaller CCK-4 (7, 8). In the CNS, CCKBR has been implicated in anxiety, depression, schizophrenia, depression, and opioid analgesia. In the stomach, CCK2 mediates gastrin-stimulated gastric acid secretion.
M00154
¥85000

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Synonyms

CCK2 receptor, CCK-B, CCK-B/gastrin, CCKB/gastrin, CCKB, CHOLREC, Cck2r, CCK(B) receptor, CCK-BR, CCK2 receptor, CCK2-R, Cholecystokinin B receptor, cholecystokinin-2 receptor, gastrin/cholecystokinin type B receptor, CCK-B/gastrin receptor, CCK2/gastrin, CCKR-2

Applications Functional assay for CCKB receptor

Storage Liquid nitrogen immediately upon delivery
Species Human

Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400ml Zeocin (Cat. #R250-01, Life Technologies)

  • CHO-K1/CCKB Stable Cell Line
  • CHO-K1/CCKB Stable Cell Line

    Figure 1: CCK8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CCKB cells. The cells were loaded with Calcium-4 prior to stimulation with the agonist, CCK8. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of CCK8 (Mean ± SD, n = 2). The EC50 of CCK8 on this cell was 0.95 nM.
    Note:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration.
    Y is the response and starts at Bottom and goes to Top with a sigmoid shape.


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