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CHO-K1/OXTR Stable Cell Line

Figure 1: oxytocin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OXTR cells. The cells were loaded with Calcium-4 prior to stimulation with OXTR receptor agonist, oxytocin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of oxytocin (Mean ± SD, n = 2). The EC50 of oxytocin on CHO-K1/OXTR cells was 0.68 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/OXTR Stable Cell Line

Oxytocin is an abundant neuropeptide that exerts a wide spectrum of central and peripheral effects. In the context of human reproduction, oxytocin promotes uterine contractions and lactation. It is the most commonly used drug for labor induction. Oxytocin receptors are expressed in the uterus and in mammary glands, where they mediate functions related to partutition, such as contraction of the uterine myometrium during labor and milk letdown. In addition, oxytocin receptors are expressed in a variety of other peripheral tissues and in the brain, where oxytocin mediates a variety of functions. OXTR antagonists may be promising candidates to prevent preterm labor and dysmenorrheal and its agonists may also be useful for treatment of psychiatric illnesses such as anxiety, drug abuse, sexual dysfunctions, eating disorders, and autism.
M00195
¥85000

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Synonyms

Human Recombinant Oxytocin Receptor Stable Cell Line

Applications Functional assay for OXTR receptor

Storage Liquid nitrogen immediately upon delivery
Species Human

Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12 K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco)

  • CHO-K1/OXTR Stable Cell Line
  • CHO-K1/OXTR Stable Cell Line

    Figure 1: oxytocin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/OXTR cells. The cells were loaded with Calcium-4 prior to stimulation with OXTR receptor agonist, oxytocin. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of oxytocin (Mean ± SD, n = 2). The EC50 of oxytocin on CHO-K1/OXTR cells was 0.68 nM.
    Note:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration.
    Y is the response and starts at Bottom and goes to Top with a sigmoid shape.


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