Figure 1. H+-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GPR68/Gα15. The cells were loaded with Calcium-4 prior to being stimulated with H+. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (0.4 log dilution) of H+ (Mean ± SD, n = 2). The EC50 of H+ on this cell was pH 5.6.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom
CHO-K1/GPR68/Gα15 Stable Cell Line
The GPR68 is a proton-sensing receptor involved in pH homeostasis. A study revealed that in osteosarcoma cells and primary human osteoblast precursors which expression of GPR68 exhibit strong PH-dependent inositol phosphate formation.
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