Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/5-HT4/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with 5-HT. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of 5-HT (Mean ± SEM, n = 3). The EC50 of 5-HT on this cell was 8.35 nM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom
CHO-K1/5-HT4/Gα15 Stable Cell Line
5-HT4 is a human serotonin G protein-coupled receptor. Several studies suggested that intact 5-HT systems are important determinants of sensitivity and/or tolerance to ethanol-induce ataxia hypothermia. Attenuated response to stress and novelty and hypersensitivity to seizures in 5-HT receptor knock-out mice. Neuropsychiatric disorders such as anxiety, depression, migraine, vasospasm and epilepsy may involve different subtypes of the 5-HT receptor.
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