目录产品 » 稳定细胞系 » Human Recombinant PD-L1 Stable Cell Line

Figure 1: FACS analysis of cell surface expression of PD-L1 on CHO-K1 cells. The CHO-K1/PD-L1 cells (red) and the negative control CHO-K1 cells (green) were probed using Anti-Human PD-L1 (GenScript) and Alexa Fluor™ 488 goat anti-human IgG (H+L) (Cat. No. A11013, Invitrogen).

CHO-K1/PD-L1 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress Homo sapiens programmed death-ligand 1 (PD-L1) on the cell surface. The surface expression is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against PD-L1 or to measure binding affinity between PD-L1 and anti-PD-L1 antibodies.

产品编号	M00543
规格	2 vials 定制报价
数量
价格	￥85000


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产品概述

Product Description	Recombinant CHO-K1 cells stably overexpress Homo sapiens programmed death-ligand 1 (PD-L1) on the cell surface. The surface expression is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against PD-L1 or to measure binding affinity between PD-L1 and anti-PD-L1 antibodies.
Applications	Cell-based binding assay or use as immunogen.

产品简介

Expressed Gene	Codon Optimized from NM_014143.3; no expressed tags
Target Protein	NP_054862.1
Host Cell	CHO-K1
Quantity	2 vials of frozen cells (> 1×10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No.LT07-318, Lonza).
Stability	Stable through more than 15 passages with no significant changes in assay performance or expression profile.

培养条件

Culture Properties	Adherent
Freeze Medium	95% Culture Medium, 5% (V/V) DMSO (Cat. No.D2650, Sigma)
Complete Growth Medium	Ham's F-12K (Kaighn's) Meidum (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium	Ham’s F-12K (Kaighn’s) Meidum (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No.A11138-03, Gibco)

技术背景

Gene Synonyms

CD274; B7-H; B7H1; PDCD1L1; PDCD1LG1; PDL1

Background

Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8⁺ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2.

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