目录产品 » 稳定细胞系 » Human Recombinant CD16A 158V Stable Cell Line

Figure 1: FACS analysis of cell surface expression of human CD16A 158V on CHO-K cells.
CHO-K1/CD16A 158V cells (blue) and negative control CHO-K1 cells (red) were stained with PE-Cy™5 Mouse Anti-Human CD16 antibody (Cat. No. 555408, BD Bioscience).

CHO-K1/CD16A 158V Stable Cell Line

Recombinant CHO-K1 cells stably express low affinity immunoglobulin gamma Fc region receptor III-A (FcγRIIIa / CD16A 158V) complex on the cell surface, including 1 alpha chain and 2 gamma chains. The surface expression of CD16A is validated by FACS analysis. This stable cell line product is designed for measuring the binding affinity and stability of the Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD16A 158V. GenScript also offers a stable cell line expressing the CD16A of 158F allotype (Cat. No. M00598) for FcγRIIIa polymorphism study.

产品编号	M00597
规格	2 vials 定制报价
数量
价格	￥85000


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产品概述

Product Description

Applications

Cell-based binding assay; use as immunogen for antibody development

产品简介

Expressed Gene	Codon optimized from NM_000569.6 (for FCGR3A); codon optimized from NM_004106.2 (for FCER1G); no expressed tags
Host Cell	CHO-K1
Quantity	Two vials of frozen cells (>1×10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

培养条件

Culture Properties	Adherent
Freeze Medium	95% Culture Medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco), 200μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)

技术背景

Gene Synonyms

FCGR3A, FCG3, FCGR3 (for Fc Fragment of IgG Receptor IIIa); FCER1G, FCRG (for Fc fragment of IgE receptor Ig)

Background

CD16 is a cluster of differentiation molecule found on the surface of natural killer cells, monocytes and macrophages. Antibodies against CD16 can be used to isolate populations of these cells using fluorescent-activated cell sorting or magnetic-activated cell sorting. CD16 has been identified as Fc receptors FcγRIIIa (CD16A) and FcγRIIIb (CD16B). The two isoforms of the CD16A stimulatory receptor found on natural killer (NK) cells and macrophages differ by one amino acid at codon 158 (V/F) and are important for antibody-dependent cell-mediated cytotoxicity (ADCC). The high affinity 158 V/V homodimer of CD16a is present on approximately 20% of the population and the lower affinity F/F homodimer and F/V heterodimer are present in the remaining 80%.
Human FcγRIIIA (CD16A) is a low-affinity receptor for the Fc portion of IgG. It is a protein complex composed of an alpha chain encoded by FCGR3A (Fc Fragment of IgG Receptor IIIa) and two gamma chains encoded by FCER1G (Fc Fragment of IgE Receptor Ig).

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