目录产品 » 稳定细胞系 » Human Recombinant CD16B Stable Cell Line

Figure 1: FACS analysis of cell surface expression of CD16B NA1 on CHO-K1/CD16B NA1 cells.
The CHO-K1/CD16B NA1 cells (blue) and the negative control CHO-K1 cells (blue) were stained with PE anti-human CD16 antibody (Cat. No. 302008, BioLegend).

CHO-K1/CD16B NA1 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human Fc fragment of IgG receptor IIIb (FcγRIIIb / CD16B NA1). The surface expression of CD16B NA1 is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, or antibody based biologics that bind with CD16B during biologics research and development.

产品编号	M00602
规格	2 vials 定制报价
数量
价格	￥85000


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产品概述

Product Description

Applications

Cell-based binding assay; use as immunogen for antibody development

产品简介

Expressed Gene	Codon optimized from CCDS41433.1; no expressed tags
Target Protein	NP_000561.3
Host Cell	CHO-K1
Quantity	2 vials of frozen cells (>1×10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

培养条件

Culture Properties	Adherent
Freeze Medium	95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco)
Culture Medium	Ham's F-12K (Kaighn's) (Cat. No.21127-022, Gibco), 10% FBS (Cat. No.10099-141, Gibco), 8 μg/ml Puromycin (Cat. No. A11138-03, Gibco)

技术背景

Gene Synonyms

CD16; FCG3; CD16b; FCGR3; FCR-10; FCRIII; FCRIIIb

Background

CD16 has been identified as FcγRIIIa (CD16A) and FcγRIIIb (CD16B) which are similar to each other. CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells. CD16B exists as a monomeric glycosylphosphatidylinositol (GPI)-anchored form in neutrophils [1]. CD16B (FcγRIIIb) exists in two allelic forms, NA1 and NA2, [2-3]. The polymorphism of CD16B exhibits different binding affinities for IgG1 and IgG3 subclasses [3]. The different allelic versions are also demonstrated to be associated with susceptibility to diseases like idiopathic pulmonary fibrosis [4] and systemic lupus [5].

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