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dsDNA_TRAC_EGFP_CTS

dsDNA_TRAC_EGFP_CTS
KIOK8
询价

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Description dsDNA_TRAC_EGFP_CTS HDR donor template is high purity GenWandTM closed-end linear dsDNA designed for inserting EGFP into targeted TRAC locus. The resulting EGFP expression will be placed under the endogenous TCR promoter. 

Advantages of GenWandTM dsDNA donor template:
✔ Covalently closed ends protection for better accuracy
✔ More suitable for scale up
✔ High purity and sequence verified
✔ Research to GMP grade
Learn More>>> //www.genscript.com/genwand-double-stranded-dna-synthesis-service.html

This design also includes Cas9 Targeting Sequence (CTS) for improved gene knock-in efficiency.  CTS allows for the co-delivery of donor DNA and sgRNA/Cas9 complex into the nucleus target gene site, therefore increasing HDR rate. More info for CTS design can be found in this paper collaborated with Dr. Marson at Gladstone and UCSF. 

This dsDNA HDR donor template needs to work in combination with SafeEdit Rab11a sgRNA and Cas9 protein. 

[Reference] Shy, et al. Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturing. bioRxiv 2021.09.02.458799.
Quantity 50ug
Storage Condition Stable for at least 12 months at -20°C. Avoid repeated freeze-thaw cycles.
Quality Specifications
Item Specifications
Purity (Agarose gel electrophoresis) Single band
Sequence accuracy (Sanger sequencing) 100% sequence alignment
Optical density (Spectrophotometry) 260 nm/230 nm ≥ 2.0; 260 nm/280 nm = 1.8~2.0
Bioburden No colony formation
Endotoxin (Qualitative TAL assay) ≤ 10 EU/mg
Protein Residue (Micro BCA Protein Assay Kit ) ≤50ug/mg

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