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CytoSinct™ CD8 Nanobeads, human

The CytoSinct™ CD8 Nanobeads (human) are used for separating CD8+ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products or single cell suspension based on the surface expression of human CD8. The surface of Nanobeads are labeled with anti-human CD8 monoclonal antibody. To begin the isolation, Nanobeads are added to the cells. The CD8 antibodies preconjugated to the Nanobeads can bind the target cells expressing CD8 on cell surface. The cells/beads mixed suspension is loaded onto a CytoSinct™ Column which is effectively magnetized by an external magnetic field from CytoSinct™ Magnet, or other compatible cell isolation columns and magnets. The NanoBeads-labeled CD8+ cells are retained within the column and enriched during the wash step when Isolation Buffer is used to flush out the CD8- cells. After removing magnetic field, the target CD8+ cells can be easily eluted from the column.
L00864
¥1875

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Description

The CytoSinct CD8 Nanobeads (human) are used for separating CD8+ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products or single cell suspension based on the surface expression of human CD8. The surface of Nanobeads are labeled with anti-human CD8 monoclonal antibody. To begin the isolation, Nanobeads are added to the cells. The CD8 antibodies preconjugated to the Nanobeads can bind the target cells expressing CD8 on cell surface. The cells/beads mixed suspension is loaded onto a CytoSinct Column which is effectively magnetized by an external magnetic field from CytoSinct Magnet, or other compatible cell isolation columns and magnets. The NanoBeads-labeled CD8+ cells are retained within the column and enriched during the wash step when Isolation Buffer is used to flush out the CD8- cells. After removing magnetic field, the target CD8+ cells can be easily eluted from the column.

Note

For research use only. Not for diagnostic use. Not for resale. Genscript will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Reactivity Human
Components Nanobeads conjugated to monoclonal mouse anti-human CD8 antibodies (isotype: mouse IgG1) under optimal conditions.
Volume
L00864-0.5500 µLFor 5 × 108 total MNCs
L00864-11 mLFor 1 × 109 total MNCs
Formulation Supplied in buffer containing stabilizer
Storage Nanobeads conjugated to monoclonal mouse anti-human CD8 antibodies (isotype: mouse IgG1) under optimal conditions.

Positive selection or depletion of CD8+ T cells from leukapheresis, PBMC or cell cultures.  Isolated CD8+ T cells can be used for culture and expansion, flow cytometry, T cells functional assays such as cytokine production cytotoxicity, coculture experiments of CD8+ T cells with dendritic cells or B cells for evaluation of the activation of antigen-presenting cells, and translational research.


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