The CytoSinct™ Streptavidin Nanobeads are used for cell separation from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products or single cell suspension in combination with biotinlated antibody or ligand. The surface of Nanobeads are labeled with streptavidin protein. To begin the isolation, the cells are stained with a biotinylated primary antibody or ligand. Subsequently, Nanobeads are added to the cells. The streptavidin preconjugated to the Nanobeads can bind the biotinlated ligand on cell surface. The cells/beads mixed suspension is loaded onto a CytoSinct™ Column which is effectively magnetized by an external magnetic field from CytoSinct™ Magnet, or other compatible cell isolation columns and magnets. The Nanobeads-labeled cells are retained within the column and enriched during the wash step when Isolation Buffer is used to flush out the cells. After removing magnetic field, the target cells can be easily eluted from the column.