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Enceed™ T Cell Activation, Human

Enceed™ T Cell Activation, Human

Enceed™ T Cell Activation, Human

T cells enriched by CytoSinct™ CD4 and CytoSinct™ CD8 nanobeads were activated by Enceed™ T cell Activation reagent. After 2 days, T cells were stained with CD25-PE and CD69-APC antibodies. Expression of CD25 and CD69 was analyzed by flow cytometry (Figure A).

Enceed™ T Cell Activation, Human

T cells stained with carboxy fluorescein succinimidyl ester (CFSE) were stimulated by Enceed™ T cell Activation. After 7 days, proliferation was tested by flow cytometry.

Enceed™ T cell Activation, human

The Enceed™ T cell Activation reagent induces T cell activation and expansion in enriched T cells or PBMCs. The Enceed™ T cell Activation reagent is composed of biodegradable matrix-coated nanoparticles conjugated with mouse anti-human CD3 and mouse anti-human CD28 antibodies. The small sized particulate structure of the activation reagent enables its easy removal by media washing or centrifugation.
L00899
¥2100

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Description

The Enceed™ T cell Activation reagent induces T cell activation and expansion in enriched T cells or PBMCs. The Enceed™ T cell Activation reagent is composed of biodegradable matrix-coated nanoparticles conjugated with mouse anti-human CD3 and mouse anti-human CD28 antibodies. The small sized particulate structure of the activation reagent enables its easy removal by media washing or centrifugation. 
The recommended titer of 1:200 has been found to achieve efficient stimulation of majority of T cell subsets. For special applications, the optimal stimulation titer should be determined experimentally. Excessive amount of the Enceed T cell Activation reagent may result in over-activation of T cells risking activation-induced cell death. During cell expansion using the Enceed T cell Activation reagent, the cells take up to 13 days after reagent application to show optimal expansion. Restimulation after 13 days can be performed for longer cultivation. The Enceed T cell Activation reagent can be used together with cytokines such as human IL-2, IL-15, and IL-7 for more efficient activation. 

Note

For research use only. Not intended for human and animal therapeutic or diagnostic use.

Reactivity Human
Formulation Biodegradable matrix-coated nanoparticles conjugated with anti-CD3 and anti-CD28 antibodies supplied in phosphate buffered-saline (PBS), containing Human Serum Albumin (HSA), pH 7.0 - 7.4.
Storage Store at 2-8 °C. Do not freeze.

Enceed T cell Activation reagent has been developed for in vitro activation and expansion of enriched T cell populations or cells from hematological cell populations (e.g. PBMCs). 

  • Enceed™ T Cell Activation, Human
  • Enceed™ T Cell Activation, Human

  • Enceed™ T Cell Activation, Human
  • Enceed™ T Cell Activation, Human

  • Enceed™ T Cell Activation, Human
  • Enceed™ T Cell Activation, Human

    T cells enriched by CytoSinct™ CD4 and CytoSinct™ CD8 nanobeads were activated by Enceed™ T cell Activation reagent. After 2 days, T cells were stained with CD25-PE and CD69-APC antibodies. Expression of CD25 and CD69 was analyzed by flow cytometry (Figure A).

  • Enceed™ T Cell Activation, Human
  • Enceed™ T Cell Activation, Human

    T cells stained with carboxy fluorescein succinimidyl ester (CFSE) were stimulated by Enceed™ T cell Activation. After 7 days, proliferation was tested by flow cytometry.


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