The Enceed™ T cell Activation reagent induces T cell activation and expansion in enriched T cells or PBMCs. The Enceed™ T cell Activation reagent is composed of biodegradable matrix-coated nanoparticles conjugated with mouse anti-human CD3 and mouse anti-human CD28 antibodies. The small sized particulate structure of the activation reagent enables its easy removal by media washing or centrifugation. 
The recommended titer of 1:200 has been found to achieve efficient stimulation of majority of T cell subsets. For special applications, the optimal stimulation titer should be determined experimentally. Excessive amount of the Enceed™ T cell Activation reagent may result in over-activation of T cells risking activation-induced cell death. During cell expansion using the Enceed™ T cell Activation reagent, the cells take up to 13 days after reagent application to show optimal expansion. Restimulation after 13 days can be performed for longer cultivation. The Enceed™ T cell Activation reagent can be used together with cytokines such as human IL-2, IL-15, and IL-7 for more efficient activation.