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Anti-DYKDDDDK Affinity Resin Easy

Figure 1. Flow chart of purification of DYKDDDDK-tagged protein

Anti-DYKDDDDK Affinity Resin Easy

Figure 2. Flow chart of immunoprecipitation of DYKDDDDK-tagged protein

Anti-DYKDDDDK Affinity Resin Easy

Lanes:
1. Eluate purified by GenScript resin
2. Eluate purified by Competitor-S resin
3. DYKDDDDK-tagged protein (~55 kDa)
4. Whole cell lysate
The red arrows indicate the bands of the target protein.

Figure 3. The DYKDDDDK-tagged proteins from cell lysates of various expression systems were captured by GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) and Competitor-S resin, respectively. The captured proteins were eluted by 0.4 mg/ml MDYKDHDGDYKDHDIDYKDDDDK peptide in TBS, and then were analyzed by SDS-PAGE. The results showed that GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) efficiently captured DYKDDDDK-tagged proteins from cell lysates of various expression systems. Especially, GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) significantly outperforms the competing product when purifying the target protein from yeast and E. coli lysates.

Anti-DYKDDDDK Affinity Resin Easy

Figure 4. Purify the DYKDDDDK-tagged protein by GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907). The target protein was eluted by 0.1 mg/ml DYKDDDDK peptide in TBS. Then, the resin was regenerated by the regeneration solution for repeated use. The coefficient of variation (CV) of the amount of eluted target protein was less than 20% for 10 purification processes in total, indicating that GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) is capable of multiple repeated use with relatively high consistency when eluted by DYKDDDDK peptide.

Anti-DYKDDDDK Affinity Resin Easy

Figure 5. Purify the DYKDDDDK-tagged protein by GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907). The protein was eluted by 0.4 mg/ml MDYKDHDGDYKDHDIDYKDDDDK peptide in TBS. Then, the resin was regenerated by the regeneration solution for repeated use. The coefficient of variation (CV) of the amount of eluted target protein was less than 20% among 9 purification processes in total, indicating that GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) is capable of multiple repeated use with relatively high consistency as well when eluted by MDYKDHDGDYKDHDIDYKDDDDK peptide.

Anti-DYKDDDDK Affinity Resin Easy

Lanes:
1/3/5. IP Eluate by GenScript resin
2/4/6. IP Eluate by Competitor-S resin
7. DYKDDDDK-tagged protein (~55 kDa)
8. Whole HEK293 lysate

Figure 6. Different amounts (5/10/25 ng) of DYKDDDDK-tagged protein (~55 kDa) were spiked to 0.5 ml of HEK293 lysis buffer, and captured by GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) and Competitor-S resin, respectively. After washing step, the resin (containing the target protein) was then eluted by SDS-PAGE sample loading buffer, followed by a Western Blotting analysis of the eluted protein. The data showed that GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) outperforms the competing product in sensitivity for immunoprecipitation applications.

Anti-DYKDDDDK Affinity Resin Easy

Lanes:
1. HA-tagged protein (~20 kDa)
2. Competitor-S resin
3. Competitor-T resin
4. Competitor-ST resin
5. GenScript resin

Figure 7. 10 μg of HA-tagged protein was incubated with GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No.L00907) and other competitor products, respectively. The proteins were eluted by SDS-PAGE sample loading buffer, then analyzed by Western blotting. Data showed that GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) exhibited extremely low nonspecific binding to the HA-tagged protein and outperforms the competing products.

Anti-DYKDDDDK Affinity Resin Easy

GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.
L00907
¥3653

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Product Description The DYKDDDDK tag is an octapeptide tag that can be added to proteins through the recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the target protein, facilitating the detection and purification of recombinant proteins. GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.

Particle concentration 50% slurry in PBS, 0.02% Sodium azide
Application Purification; Immunoprecipitation
Matrix 4% cross-linked agarose
Ligand Monoclonal antibody against DYKDDDDK tag
Particle size 45-165 μm
Binding Capacity ~1 mg DYKDDDDK-tagged protein (~55 kDa) per ml settled resin
Storage & Stability Store at 2°C~8°C. Do not store at or below 0°C to avoid freezing the resin.

  • Anti-DYKDDDDK Affinity Resin Easy
  • Anti-DYKDDDDK Affinity Resin Easy

    Figure 1. Flow chart of purification of DYKDDDDK-tagged protein

  • Anti-DYKDDDDK Affinity Resin Easy
  • Anti-DYKDDDDK Affinity Resin Easy

    Figure 2. Flow chart of immunoprecipitation of DYKDDDDK-tagged protein

  • Anti-DYKDDDDK Affinity Resin Easy
  • Anti-DYKDDDDK Affinity Resin Easy

    Lanes:
    1. Eluate purified by GenScript resin
    2. Eluate purified by Competitor-S resin
    3. DYKDDDDK-tagged protein (~55 kDa)
    4. Whole cell lysate
    The red arrows indicate the bands of the target protein.

    Figure 3. The DYKDDDDK-tagged proteins from cell lysates of various expression systems were captured by GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) and Competitor-S resin, respectively. The captured proteins were eluted by 0.4 mg/ml MDYKDHDGDYKDHDIDYKDDDDK peptide in TBS, and then were analyzed by SDS-PAGE. The results showed that GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) efficiently captured DYKDDDDK-tagged proteins from cell lysates of various expression systems. Especially, GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) significantly outperforms the competing product when purifying the target protein from yeast and E. coli lysates.

  • Anti-DYKDDDDK Affinity Resin Easy
  • Anti-DYKDDDDK Affinity Resin Easy

    Figure 4. Purify the DYKDDDDK-tagged protein by GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907). The target protein was eluted by 0.1 mg/ml DYKDDDDK peptide in TBS. Then, the resin was regenerated by the regeneration solution for repeated use. The coefficient of variation (CV) of the amount of eluted target protein was less than 20% for 10 purification processes in total, indicating that GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) is capable of multiple repeated use with relatively high consistency when eluted by DYKDDDDK peptide.

  • Anti-DYKDDDDK Affinity Resin Easy
  • Anti-DYKDDDDK Affinity Resin Easy

    Figure 5. Purify the DYKDDDDK-tagged protein by GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907). The protein was eluted by 0.4 mg/ml MDYKDHDGDYKDHDIDYKDDDDK peptide in TBS. Then, the resin was regenerated by the regeneration solution for repeated use. The coefficient of variation (CV) of the amount of eluted target protein was less than 20% among 9 purification processes in total, indicating that GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) is capable of multiple repeated use with relatively high consistency as well when eluted by MDYKDHDGDYKDHDIDYKDDDDK peptide.

  • Anti-DYKDDDDK Affinity Resin Easy
  • Anti-DYKDDDDK Affinity Resin Easy

    Lanes:
    1/3/5. IP Eluate by GenScript resin
    2/4/6. IP Eluate by Competitor-S resin
    7. DYKDDDDK-tagged protein (~55 kDa)
    8. Whole HEK293 lysate

    Figure 6. Different amounts (5/10/25 ng) of DYKDDDDK-tagged protein (~55 kDa) were spiked to 0.5 ml of HEK293 lysis buffer, and captured by GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) and Competitor-S resin, respectively. After washing step, the resin (containing the target protein) was then eluted by SDS-PAGE sample loading buffer, followed by a Western Blotting analysis of the eluted protein. The data showed that GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) outperforms the competing product in sensitivity for immunoprecipitation applications.

  • Anti-DYKDDDDK Affinity Resin Easy
  • Anti-DYKDDDDK Affinity Resin Easy

    Lanes:
    1. HA-tagged protein (~20 kDa)
    2. Competitor-S resin
    3. Competitor-T resin
    4. Competitor-ST resin
    5. GenScript resin

    Figure 7. 10 μg of HA-tagged protein was incubated with GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No.L00907) and other competitor products, respectively. The proteins were eluted by SDS-PAGE sample loading buffer, then analyzed by Western blotting. Data showed that GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) exhibited extremely low nonspecific binding to the HA-tagged protein and outperforms the competing products.


For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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