| Product Description |
MonoRab™
Rabbit Anti-VHH Microbeads are superparamagnetic beads with a diameter of 1.5
µm. These beads are coated with anti-VHH antibodies and can be used to separate
VHH+cells from fresh or frozen camelid peripheral blood mononuclear
cells (PBMCs) or single cell suspensions based on the surface expression of
VHH. To initiate the isolation process, the beads are added to the cell sample
in a tube. After a brief incubation, the anti-VHH antibodies that are
preconjugated to the beads will bind to the target cells that express VHH on
their cell surface. The bead-bound cells can then be separated from the rest of
the sample using a magnet. MonoRab™ Rabbit Anti-VHH Microbeads will provide
excellent recovery of high-purity, viable cells for downstream molecular research. |
| Capacity |
for up to 5×108 camelid PBMCs every 500 μL microbeads.
|
| Reactivity |
Alpaca, Llama and
Camel VHH+ cells
|
| Ligand |
Anti-VHH antibody
|
| Beads Diameter |
1.5 μm
|
| Presentation |
10 mg/mL, supplied in PBS (pH 7.2),
containing 1 mg/mL BSA, 2 mM EDTA and 0.03% Proclin 300. |
| Application |
Positive selection or
depletion of VHH+ cells from PBMCs or cell cultures. Isolated VHH+ cells can be used in downstream
molecular studies.
|
| Storage |
Store at 2-8 °C for up to 12 months. Do not freeze
the beads.
|
VHH+ cells were isolated from camelid PBMCs using MonoRab™ Rabbit Anti-VHH Microbeads. VHH+ cells were stained with MonoRab™ Rabbit Anti-Camelid VHH Cocktail [iFluor 647] (GenScript, A02019).
For
research use only. Not intended for human and animal therapeutic or diagnostic
use.
