| Species |
Human |
| Protein Construction |
4-1BB (Glu18-Ser184)
Accession # Q07011 |
|
| Purity |
> 97% as analyzed by SDS-PAGE
> 97% as analyzed by HPLC |
| Endotoxin Level |
< 1 EU/μg of protein by LAL method |
| Biological Activity |
Fully biologically active when compared to standard. The biological activity is determined by its inhibitory effect of IL-8 production using human peripheral blood mononuclear cells. About 90 % of inibition was seen using a concentration of 1.0 μg for both 4-1BB Ligand and 4-1BB Receptor. |
| Expression System |
E. coli |
| Theoretical Molecular Weight |
17.7 kDa |
| Formulation |
Lyophilized from a 0.2 μm filtered solution in 10 mM PB, pH 8.0, 150 mM NaCl. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
| Target Background |
4-1BB(CD137) is a member of the tumor necrosis factor (TNF) receptor family. Mature human 4-1BB consists of a 163 amino acid extracellular domain (ECD) with four TNFR cysteine‑rich repeats, a 27 aa transmembrane segment, and a 42 aa cytoplasmic domain; 4-1BB (CD137) is expressed as a disulfide-linked homodimer on various populations of activated T cell including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts. It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells. This interaction co-stimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell. It can also enhance the activation-induced cell death of repetitively stimulated T cells. |
| Synonyms |
4-1BB; TNFRSF9; CD137 antigen; T-cell antigen ILA |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
