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Enterokinase, His, Bovine

Lane 1: 2 μg of Enterokinase, His, Bovine, non-reducing (NR)
Lane 2: 2 μg of Enterokinase, His, Bovine, reducing (R)
> 95% as analyzed by SDS-PAGE

Enterokinase, His, Bovine

Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.
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Description

Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. 
Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. 
Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.
Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques at GenScript.

Synonyms

Enteropeptidase, ENTK, PRSS7

Note

For research use only

Components 1. 100 IU(or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
2. 100μg Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0)
3. 3.6 ml EK Dilution/Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl₂, 50% glycerol)
4. 1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl₂)
Source P. pastoris
Species Bovine

Molecular Weight ~40 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE analyses.
Endotoxin Level < 1.0 EU/μg, determined by LAL method.
Biological Activity 5 IU/μl.
Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25mM Tris-HCl, pH 8.0.
Formulation Sterile liquid solution contians 20mM Tris, 200mM NaCl, 2mM CaCl2, 50% glycerol, pH 7.4.
Storage & Stability Recombinant Bovine Enterokinase (rbEK) remains stable up to 1 year at -20°C from date of receipt. It will remain stable at 37°C for one week without losing any activity.Please avoid freeze-thaw cycles.

  • Enterokinase, His, Bovine
    • Enterokinase, His, Bovine

    Lane 1: 2 μg of Enterokinase, His, Bovine, non-reducing (NR)
    Lane 2: 2 μg of Enterokinase, His, Bovine, reducing (R)
    > 95% as analyzed by SDS-PAGE


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