GenScript product showed better activity compared to competitor P

Biological Activity
FGF-basic, Human (Cat. No. Z03116) stimulates cell proliferation of Balb/3T3 cells. The ED₅₀ for this effect is less than 0.25ng/mL.

2 μg of FGF-basic, Human (Cat. No. Z03116) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

FGF-basic (154aa), Human

Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, bFGF has the β trefoil structure. In vivo, bFGF is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. bFGF regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. bFGF can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of bFGF can produce beneficial cardioprotection during acute heart injury.

产品编号	Z03116
规格	10 μg 50 μg 1 mg 定制报价
数量
价格	￥300


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产品简介

Species

Human

Protein Construction

FGF-basic (Ala135-Ser288)
Accession # P09038

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 0.25 ng/ml, measured by the cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 4.0 × 10⁶ units/mg.

Expression System

E. coli

Apparent Molecular Weight

~17.1 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

图例

GenScript product showed better activity compared to competitor P

Biological Activity
FGF-basic, Human (Cat. No. Z03116) stimulates cell proliferation of Balb/3T3 cells. The ED₅₀ for this effect is less than 0.25ng/mL.

2 μg of FGF-basic, Human (Cat. No. Z03116) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie Blue staining.

技术背景

Target Background

Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, bFGF has the β trefoil structure. In vivo, bFGF is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. bFGF regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. bFGF can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of bFGF can produce beneficial cardioprotection during acute heart injury.

Synonyms

Fibroblast Growth Factor-basic; FGF-2; BFGF; FGFB; HBGF-2; bFGF; Prostatropin

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

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FGF-basic (154aa), Human

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