ED₅₀ < 1.0 ng/ml, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 1.0 × 10⁶ units/mg.

Lane 1: 2 μg of FGF-basic, Bovine, non-reducing (NR)
Lane 2: 2 μg of FGF-basic, Bovine, reducing (R)
> 95% as analyzed by SDS-PAGE

FGF-basic, Bovine

Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.

产品编号	Z03230
规格	10 μg 50 μg 1 mg 定制报价
数量
价格	￥300


联系凯发一触即发

产品简介

Species

Bovine

Protein Construction

FGF-basic (Pro10-Ser155)
Accession # P03969

Purity

> 95% as analyzed by SDS-PAGE

Endotoxin Level

< 0.2 EU/μg of protein by gel clotting method

Biological Activity

ED₅₀ < 1.0 ng/ml, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 1.0 × 10⁶ units/mg.

Expression System

E. coli

Apparent Molecular Weight

~16.4 kDa, on SDS-PAGE under reducing conditions.

Formulation

Lyophilized after extensive dialysis against PBS.

Reconstitution

It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in PBS up to 100 μg/ml.（It is recommended to increase the concentration of NaCl in PBS to 300 mM）

Storage & Stability

Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

图例

ED₅₀ < 1.0 ng/ml, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 1.0 × 10⁶ units/mg.

Lane 1: 2 μg of FGF-basic, Bovine, non-reducing (NR)
Lane 2: 2 μg of FGF-basic, Bovine, reducing (R)
> 95% as analyzed by SDS-PAGE

技术背景

Target Background

Synonyms

Fibroblast Growth Factor-basic; FGF-2; BFGF; FGFB; HBGF-2; bFGF; Prostatropin

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.