| Species |
Human |
| Protein Construction |
FGF-10 (Leu40-Ser208)
Accession # O15520 |
|
| Purity |
> 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 20.0 ng/ml, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0 × 104 units/mg. |
| Expression System |
E. coli |
| Apparent Molecular Weight |
~19.3 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
Lane 1: 2μg of FGF-10, Human, non-reducing(NR)
Lane 2: 2μg of FGF-10, Human, reducing(R)
> 95% as analyzed by SDS-PAGE
| Target Background |
Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in the lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares homology with KGF and receptor binding to FGFR2-IIIb. However, while KGF induces proliferation and differentiation of various epithelial cells, FGF-10 promotes budding and branching morphogenesis during the multi-organ development via mesenchymal-epithelial cell interactions. FGF-10 is critical for lung and limb development, and is regulated by Shh during early development. |
| Synonyms |
Fibroblast Growth Factor-10; FGFA; Keratinocyte growth factor-2 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
