| Species |
Mouse |
| Protein Construction |
MIP-2 (Ala28-Asn100)
Accession # P10889 |
|
| Purity |
> 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
The EC50 value of Mouse MIP-2/CXCL2 on Ca2+ mobilization assay in CHO-K1/mCXCR2 (mouse CXCR2 stably expressed in CHO-K1 cells) is less than 200.0 ng/ml. |
| Expression System |
HEK 293 |
| Apparent Molecular Weight |
~7.8 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background |
Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also referred to as macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2). CXCL2 is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. CXCL2’s amino acid sequence is 90% identical to the amino acid sequence of related chemokine, CXCL1. CXCL2 signals through the CXCR2 receptor. |
| Synonyms |
CINC-3; GRO beta; MGSA-beta; MIP-2 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
