Species |
SARS-CoV-2 |
Protein Construction |
S1 protein (Val16-Arg685) Accession # P0DTC2 |
Poly-His |
N-term |
C-term |
|
Purity |
> 90% as analyzed by SDS-PAGE |
Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
Biological Activity |
SARS-CoV-2 Spike protein (S1) can bind with Human ACE2 in functional ELISA assay. |
Expression System |
293 Cells |
Theoretical Molecular Weight |
79 kDa |
Formulation |
Supplied as a solution in PBS pH 7.2 containing 10% glycerol. |
Concentration |
Please refer to the COA for the specific lot. |
Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -20°C or below. Avoid repeated freeze-thaw cycles. |
Western blot analysis of SARS-CoV-2 S1 protein (His- tagged) (GenScript, Z03485) using S1 Antibody(GenScript, A02053).
Lane 1: 10 ng of Z03485
Lane 2: 5ng of Z03485
Western blot analysis of SARS-CoV-2 S1 protein (His- tagged) (GenScript, Z03485) using His Antibody(GenScript, A00186).
Lane 1: 10 ng of Z03485
Lane 2: 5ng of Z03485
Immobilized SARS-CoV-2 S1 protein(His Tag) (Cat. No. Z03485) at 1 μg/mL can bind ACE-2 Fc Chimera, Human (Cat. No. Z03516) with a serial dilution.
Mouse Anti-Human IgG Fc Antibody[HRP], mAb (Cat.No. A01854) is used as a secondary antibody (0.1 μg/mL).
Lane 1: 1μg of SARS-CoV-2 Spike protein (S1, His Tag), reducing(R)
> 95% as analyzed by SDS-PAGE
Target Background |
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. S protein plays an important role in the induction of neutralizing antibodies and T-cell responses, as well as protective immunity. |
Synonyms |
SARS-CoV-2 S1 protein; 2019-nCoV S1 protein |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.