Species |
SARS-CoV-2 |
Protein Construction |
Spike RBD [Arg319-Ser591 (N501Y)] Accession # P0DTC2 |
Avi |
Poly-His |
N-term |
|
C-term |
|
Purity |
> 90% as analyzed by SDS-PAGE |
Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
Biological Activity |
SARS-CoV-2 Spike protein (RBD, N501Y, Avi & His Tag) can bind with human ACE2 (Cat. No.: Z03484) in functional ELISA assay. |
Expression System |
293 Cells |
Theoretical Molecular Weight |
30 kDa |
Formulation |
Supplied as a solution in PBS, pH 7.4. |
Concentration |
Please refer to the COA for the specific lot. |
Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -20°C or below. Avoid repeated freeze-thaw cycles. |
Immobilized ACE-2 Fc Chimera, Human (Cat. No. Z03484) at 2 μg/mL can bind SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag) (Cat. No. Z03533) with a serial dilution.
THETM His Tag Antibody [HRP], mAb, Mouse (Cat.No.A00612) is used as a secondary antibody (0.2 μg/mL).
Loaded ACE-2 Fc Chimera, Human (CHO-expressed) Protein (Cat. No. Z03516) on Protein A Biosensor, can bind SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag) (Cat. No. Z03533) with an affinity constant of 2.11 nM as determined in BLI assay.
Lane 1: 1μg of SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag), reducing(R)
> 95% as analyzed by SDS-PAGE
Target Background |
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein mutation N501Y is one of six key contact residues within the receptor-binding domain (RBD) and has been identified as increasing binding affinity to human and murine ACE2. |
Synonyms |
SARS-CoV-2 SP RBD, N501Y; 2019-nCoV SP RBD, N501Y |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.