GenScript), Anti-Myc Polyclonal (18826-01; Qed Biosciences), Anti-Ub (P4D1, Sc-8017; Santa Cruz Biotechnology, Inc.), And Anti-Ha Monoclonal...">

凯发一触即发

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

A single ubiquitin is sufficient for cargo protein entry into MVBs in the absence of ESCRT ubiquitination.

J Cell Biol.. 2011-01;  192(2):229 - 242
Daniel K. Stringer and Robert C. Piper. Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA 52246, USA.
Products/Services Used Details Operation

摘要

ESCRTs (endosomal sorting complexes required for transport) bind and sequester ubiquitinated membrane proteins and usher them into multivesicular bodies (MVBs). As Ubiquitin (Ub)-binding proteins, ESCRTs themselves become ubiquitinated. However, it is unclear whether this regulates a critical aspect of their function or is a nonspecific consequence of their association with the Ub system. We investigated whether ubiquitination of the ESCRTs was required for their ability to sort cargo into the MVB lumen. Although we found that Rsp5 was the main Ub ligase responsible for ubiquitination of ESCRT-0, elimination of Rsp5 or elimination of the ubiquitinatable lysines within ESCRT-0 did not affect MVB sorting. Moreove... More

关键词