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Comparative analysis of bacterial community-metagenomics in coastal Gulf of Mexico sediment microcosms following exposure to Macondo oil (MC252).

Antonie Van Leeuwenhoek.. 2014-11;  106(5):993-1009
Koo H, Mojib N, Thacker RW, Bej AK. Department of Biology, University of Alabama at Birmingham, Birmingham, AL, 35204, USA.
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摘要

The indigenous bacterial communities in sediment microcosms from Dauphin Island (DI), Petit Bois Island (PB) and Perdido Pass (PP) of the coastal Gulf of Mexico were compared following treatment with Macondo oil (MC252) using pyrosequencing and culture-based approaches. After quality-based trimming, 28,991 partial 16S rRNA sequence reads were analyzed by rarefaction, confirming that analyses of bacterial communities were saturated with respect to species diversity. Changes in the relative abundances of Proteobacteria, Bacteroidetes and Firmicutes played an important role in structuring bacterial communities in oil-treated sediments. Proteobacteria were dominant in oil-treated samples, whereas Firmicutes and Bac... More

关键词

Oil spill; Qiime; Bioinformatics; Biodegradative genes; PCR; Hydrocarbonoclastic bacteria; Pyrosequencing