凯发一触即发

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Immunocapture-selected reaction monitoring screening facilitates the development of ELISA for the measurement of native TEX101 in biological fluids.

Mol Cell Proteomics.. 2015-06;  14(6):1517-26
Korbakis D, Brinc D, Schiza C, Soosaipillai A, Jarvi K, Drabovich AP, Diamandis EP. 0
Products/Services Used Details Operation

摘要

Monoclonal antibodies that bind the native conformation of proteins are indispensable reagents for the development of immunoassays, production of therapeutic antibodies and delineating protein interaction networks by affinity purification-mass spectrometry. Antibodies generated against short peptides, protein fragments or even full length recombinant proteins may not bind the native protein form in biological fluids, thus limiting their utility. Here, we report the application of immunocapture coupled with Selected Reaction Monitoring (SRM) measurements, in the rapid screening of hybridoma culture supernatants for monoclonal antibodies that bind the native protein conformation. We produced mouse monoclonal anti... More

关键词

Antibodies*; Assay development; Immunoaffinity; Mass Spectrometry; Selected reaction monitoring; TEX101 ELISA; male infertility; native protein conformation; seminal plasma; testis-expressed sequence 101 protein