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Cartilage Derived from Bone Marrow Mesenchymal Stem Cells Expresses Lubricin.

PLoS One.. 2016-02; 
Nakagawa Y,Muneta T, Otabe K, Ozeki N, Mizuno M, Udo M, Saito R, Yanagisawa K, Ichinose S, Koga H, Tsuji K, Sekiya I.
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... in methanol for 15 minutes. Rabbit polyclonal anti-GFP antibody (1:100 dilution; A01704-100, GenScript, Piscataway, NJ) was applied to sec- tions and incubated at room temperature for 1 hour. The sections were incubated ...

摘要

OBJECTIVE:Lubricin expression in the superficial cartilage will be a crucial factor in the success of cartilage regeneration. Mesenchymal stem cells (MSCs) are an attractive cell source and the use of aggregates of MSCs has some advantages in terms of chondrogenic potential and efficiency of cell adhesion. Lubricin expression in transplanted MSCs has not been fully elucidated so far. Our goals were to determine (1) whether cartilage pellets of human MSCs expressed lubricin in vitro chondrogenesis, (2) whether aggregates of human MSCs promoted lubricin expression, and (3) whether aggregates of MSCs expressed lubricin in the superficial cartilage after transplantation into osteochondral defects in rats. METHODS:F... More

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