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Zinc finger protein 618 regulates UHRF2 (ubiquitin like with PHD and ring finger domains 2) function as a specific 5-hydroxymethylcytosine reader.

J Biol Chem.. 2016-06; 
Liu Y, Zhang B, Kuang H, Korakavi G, Lu LY, Yu X.
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... immunoprecipitation). The following antibody was purchased: anti-UHRF1 (Santa Cruz, sc- 373750), anti-UHRF2 (Santa Cruz, sc-398953), anti-α-tubulin (Genscript, A01410), anti-FLAG (Sigma, F1804), and anti-HA (Covance, MMS- 101P). ...

摘要

5-Hydroxymethylcytosine (5hmC) is an epigenetic modification that is generated by ten-eleven translocation (TET) protein-mediated oxidation of 5-methylcytosine (5mC). 5hmC is associated with transcription regulation and is decreased in many cancers including melanoma. Accumulating evidence has suggested that 5hmC is functionally distinct from 5mC. Ubiquitin-like with PHD and ring finger domains 2 (UHRF2) is the first known specific 5hmC reader that has higher affinity to 5hmC than 5mC, suggesting that UHRF2 might mediate 5hmC's function. Structural analysis has revealed the molecular mechanism of UHRF2-5hmC binding in vitro, but it is not clear how UHRF2 recognizes 5hmC in vivo In this study, we have identified... More

关键词

5-hydroxymethylcytosine (5-hmC); DNA demethylation; chromatin; molecular biology; molecular cell biology