| Products/Services Used | Details | Operation |
|---|---|---|
| ... 6. Antibodies used: Anti-CD63 mouse monoclonal (Abcam, #ab59479), Anti-Flag-M2 monoclonal(Sigma, #F1804), Anti-Avi mouse monoclonal (Genscript, #A01738), Normal mouse IgG (Upstate cell signaling solutions, #12-371), Goat antimouse secondary (10 nm gold ... |
Extracellular vesicle (EV) are tiny membranous vesicles usually <500nm in size that recently emerged as a new paradigm in human intercellular signaling. EVs have shown a promising role in development of diagnostic markers in many pathophysiological disorders. The presence of chemosensory and therapeutically relevant G protein-coupled receptors (GPCRs) on EV membranes is poorly characterized. Here, we compare different methods including ultracentrifugation and polymer-charge-based separation to isolate EVs from cell culture media and human saliva. The presence of bitter taste GPCRs (T2R4 and T2R38) and a class A GPCR angiotensin II type 1 receptor on these EVs was characterized by qPCR, ELISA, and immunotransmis... More
