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Functional protein-based nanomaterial produced in GRAS microorganism: a new platform for biotechnology.

Acta Biomater.. 2016-10; 
Cano-Garrido O, Sánchez-Chardi A, Parés S, Giró I, Tatkiewicz W, Ferrer-Miralles N, Ratera I, Natalello A, Cubarsi R, Veciana J, Bach À, Villaverde A, Arís A, Garcia-Fruitós E.
Products/Services Used Details Operation
... Bands were identified using a commercial polyclonal serum against histidine tag (#A00186-100 Genscript) and an anti-mouse secondary antibody (#170-6516, Bio-Rad). Recombinant protein yield were estimated with a standard curve with known amounts of a GFP-H6 protein. ...

摘要

Inclusion bodies (IBs) are protein-based nanoparticles formed in Escherichia coli through stereospecific aggregation processes during the overexpression of recombinant proteins. In the last years, it has been shown that IBs can be used as nanostructured biomaterials to stimulate mammalian cell attachment, proliferation, and differentiation. In addition, these nanoparticles have also been explored as natural delivery systems for protein replacement therapies. Although the production of these protein-based nanomaterials in E. coli is economically viable, important safety concerns related to the presence of endotoxins in the products derived from this microorganism need to be addressed. Lactic acid bacteria (LAB) ... More

关键词

Endotoxin-free; Functional nanomaterials; GRAS; Lactic acid bacteria; Nanoparticles