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Construction of an anti-programmed death-ligand 1 chimeric antigen receptor and determination of its antitumor function with transduced cells.

Oncol Lett. 2018-07; 
XieJiasen,ZhouZishan,JiaoShunchang,LiXia
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… Z03074-10; GenScript, Piscataway, NJ, USA), and incubated in a humidified 5% CO2 incubator at 37˚C. A total of 3-5 days later, the activated cells were resuspended and ~2 million PBMCs were transduced with pre-conditioned lentiviral particles with or without scFv-28Bz in …

摘要

A chimeric antigen receptor (CAR) is a type of fusion protein that comprises an antigen-recognition domain and signaling domains. In the present study, a programmed death-ligand 1 (PD-L1)-specific CAR, comprised of a single-chain variable fragment (scFv) derived from a monoclonal antibody, co-stimulatory domains of cluster of differentiation (CD) 28 and 4-1BB and a T-cell-activation domain derived from CD3ζ, was designed. The construction was cloned and packaged into the lentiviral vector pLVX. Flow cytometry confirmed that peripheral blood mononuclear cells were efficiently transduced and that the CAR was successfully expressed on T cells. The cytotoxicity of transduced T cells was detected using PD-L... More

关键词

A549,NCI-H358,chimeric antigen receptor,programmed death-liga