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Genome-wide analysis of endogenously expressed ZEB2 binding sites reveals inverse correlations between ZEB2 and GalNAc-transferase GALNT3 in human tumors.

Cell Oncol (Dordr). 2018-08; 
Balcik-ErcinPelin,CetinMetin,Yalim-CamciIrem,OdabasGorkem,TokayNurettin,SayanA Emre,YagciT
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… The following antibodies were used: anti-ZEB2 (home- made monoclonal antibody, clone 6E5) [7], anti-ACTB mouse monoclonal antibody (A00702) from GenScript (Piscataway, NJ, USA), rabbit polyclonal anti-GALNT3 antibody (LS-C166355) from LifeSpan BioSciences …

摘要

ZEB2 is a transcriptional repressor that regulates epithelial-to-mesenchymal transition (EMT) through binding to bipartite E-box motifs in gene regulatory regions. Despite the abundant presence of E-boxes within the human genome and the multiplicity of pathophysiological processes regulated during ZEB2-induced EMT, only a small fraction of ZEB2 targets has been identified so far. Hence, we explored genome-wide ZEB2 binding by chromatin immunoprecipitation-sequencing (ChIP-seq) under endogenous ZEB2 expression conditions.

关键词

Antibody validation,ChIP-sequencing,GALNT3,Gene regulation,Tissue expression,