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Binge alcohol alters PNPLA3 levels in liver through epigenetic mechanism involving histone H3 acetylation

Alcohol. 2017-05; 
RestrepoRicardo J, LimRobert W, KorthuisRonald J, ShuklaShivend
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… C. The protocol used for hepatocyte ChIP was adapted from earlier reports (Browne et al., 2014, Tomita et al., 2003). Magnetic beads from GenScript (Protein A/G MagBeads, GenScript catalog #L00277) were used. In addition …

摘要

The human PNPLA3 (patatin-like phospholipase domain-containing 3) gene codes for a protein which is highly expressed in adipose tissue and liver, and is implicated in lipid homeostasis. While PNPLA3 protein contains regions homologous to functional lipolytic proteins, the regulation of its tissue expression is reflective of lipogenic genes. A naturally occurring genetic variant of PNPLA3 in humans has been linked to increased susceptibility to alcoholic liver disease. We have examined the modulatory effect of alcohol on PNPLA3 protein and mRNA expression as well as the association of its gene promoter with acetylated histone H3K9 by chromatin immunoprecipitation (ChIP) assay in rat hepatocytes in vitro, ... More

关键词

Binge ethanol,Chronic-binge ethanol,Epigenetics,Histone acetylation,PN