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Pplase of Dermatophagoides farinae promotes ovalbumin-induced airway allergy by modulating the functions of dendritic cells in a mouse model.

Sci Rep. 2017-02; 
WangHui, MoLihua, XiaoXiaojun, AnShu, LiuXiaoyu, BaJinge, WuWeifang, RanPixin, YangPingchang, LiuZhi
Products/Services Used Details Operation
… Pplase proteins were purified via affinity chromatography. The endotoxin was preliminarily removed using an ion exchange column and further eliminated using the ToxinEraser Endotoxin Removal Kit (L00338, Genscript, China) …

摘要

Our previous studies revealed that many proteins in addition to the known allergens of D. farinae have not been fully characterized. We observed that Pplase did not respond to serum collected from patients sensitized to D. farinae. In a mouse model, Pplase significantly enhanced airway hyperresponsiveness (AHR) and Th2 responses induced by ovalbumin (OVA) compared with mice treated with OVA alone. Moreover, exposure to Pplase significantly increased the expression of IRF4, CD80, CD83, MHCII and TNF-α in DC2.4 cells, which was abolished in the presence of a TLR4 inhibitor. In vitro T cell polarization experiments revealed that Pplase alone could not induce T cell polarization but enhanced T cell pol... More

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